How Not To Bodyboard [Video]
Source: http://feeds.gawker.com/~r/gizmodo/full/~3/9uOnzu0Ok6c/how-not-to-body-board
nlcs josh beckett 999 plan the village detroit weather detroit weather imessage
Source: http://feeds.gawker.com/~r/gizmodo/full/~3/9uOnzu0Ok6c/how-not-to-body-board
nlcs josh beckett 999 plan the village detroit weather detroit weather imessage
DES MOINES, Iowa ? Chicago Mayor Rahm Emanuel will launch an ardent defense of President Barack Obama to Iowa Democratic activists, warning that "we just can't cut our way to prosperity."
Emanuel was scheduled to speak Saturday night at the Iowa Democratic Party's biggest annual fundraiser. Excerpts from his remarks were provided to The Associated Press in advance.
In them, Emanuel says Obama has made crucial and tough decisions based on his principles, and not to lay the groundwork for a second term in office.
"In the next four years, there will be more challenges and more crises that will determine the economic vitality of the middle class and the economic future of this country," Emanuel says. "Whose character, whose judgment do you want in that office?"
Before winning election as mayor, Emanuel was a top aide for Obama and he's been a vocal advocate for the president. Emanuel says he expects voters to focus on the character of the candidates during the upcoming campaign.
The deficit reduction debate in Washington has centered on a fight between Obama and Democrats, who argue the solution is a combination of spending cuts and tax increases. Republicans argue for sole reliance on spending cuts.
"To create true middle-class security, we can't just cut our way to prosperity," Emanuel says. "We must out-innovate, out-educate and out-build the world."
Emanuel was to be the featured speaker at the Jefferson-Jackson Day dinner in Des Moines, the largest annual fundraiser for the Iowa Democratic Party. His appearance was expected to give the party a chance to grab attention from Republican presidential candidates making their case to social conservatives just across town.
While polls show next year's election will likely be competitive, there's a debate about tactics among some Democrats. Some argue Obama benefits from a long and heated Republican primary season in which candidates are burning GOP money and attacking each other. Others warn Republicans are getting all of the media attention and that Obama will be better off when he has a single opponent with whom he can draw contrasts.
In recent weeks, Obama has sharpened his populist message as he shifts to election mode. Emanuel is echoing that theme as he seeks to rally activist Democrats.
"President Obama believes in an America where hard work pays off, where responsibility is rewarded," Emanuel says. "He believes in an America where we don't have two rule books, one for those at the top and another set for everyone else. President Obama believes in the idea that our country prospers when we're all in it together."
Obama has a long history in Iowa. His surprising win in the state's precinct caucuses four years ago launched him on the road to the White House and he easily carried the state in the 2008 general election. Polls have shown the state to be competitive in this election cycle.
boston redsox red sox law and order svu camaro zl1 bob sanders evan longoria janeane garofalo
Each time a cell divides -- and it takes millions of cell divisions to create a fully grown human body from a single fertilized cell -- its chromosomes have to be accurately divvied up between both daughter cells. Researchers at the Stowers Institute for Medical Research used, ironically enough, the single-celled organism Saccharomyces cerevisiae-- commonly known as baker's yeast -- to gain new insight into the process by which chromosomes are physically segregated during cell division.
In a study published in the Nov. 17, 2011 issue of PLoS Genetics, they demonstrate that a protein known as Mps3 not only ensures that cells have two functional spindle pole bodies, which generate the mitotic spindle apparatus that helps pull the chromosomes apart, but also that both spindle pole bodies are properly anchored in the nuclear membrane.
"When you enter mitosis, you need to have two spindle pole bodies on which you can pull the chromosomes. If you don't, the probability of errors in chromosome segregation increases exponentially," explains Jaspersen, adding that, "even small mistakes can lead to birth defects, genetic instability and cancer."
Normally, cells have only a single spindle pole body, but in preparation for cell division, the spindle pole body has to duplicate itself ? just as the genome does. "We know a whole lot about how DNA copies itself, but we don't know much about how spindle pole bodies duplicate themselves," says Jaspersen.
Unlike DNA molecules, which serve as templates for the production of identical copies, the spindle pole body is a large protein structure composed of soluble proteins and so-called integral membrane proteins, which are anchored in the nuclear envelope. The duplication process of the lone spindle pole body begins when soluble proteins coalesce on the nuclear envelope followed by their insertion into the lipid bi-layer located next to the original spindle pole body. Insertion probably requires the integral membrane proteins of the spindle pole body and results in a second functional spindle pole body.
While many genes are known to be required for spindle pole body duplication, the best studied are perhaps the conserved family of SUN-domain proteins. The SUN-protein homolog in yeast is Mps3, an integral membrane component of the spindle pole body required for early steps in the duplication process.
"Cells with little or no functional Mps3 do not divide, and have only one spindle pole body and one half of the mitotic spindle," explains Jaspersen. "We were interested in how a spindle pole body gets inserted into the nuclear envelope, what modifications of this double lipid bi-layer membrane have to occur to facilitate insertion, and what is Mps3's role in all of this?"
To better understand the function of Mps3 in spindle pole body duplication, Jaspersen's team, led by co-first authors Jennifer Friederichs and Suman Ghosh, Ph.D., mutated specific regions of the Mps3 gene and then expressed the mutated genes in yeast. For most of the mutants, mitosis seemed normal. That wasn't the case, however, with one particular novel mutant, MPS3-G186K, which has a small, "point" mutation in the so-called P-loop region.
The researchers next used high-resolution electron microscopy and various markers, including ones that can distinguish uninserted and inserted spindle pole bodies. What they saw was that, although their DNA had been duplicated, cells expressing this particular Mps3 mutant had multiple duplication defects, including blocking insertion of the spindle pole body into the nuclear envelope.
What was most striking, however, was that nearly every cell examined had nuclear membranes that were, essentially, overgrown?with two to eight layers of nuclear envelope, and multiple lobes and extensions -- instead of a simple spherical structure. Importantly, the effect seemed specific in that the other membrane-based organelles appeared normal.
"We had never seen nuclei that looked like that," recalls Jaspersen. "It suggested that Mps3 was regulating the lipid environment of the nuclear envelope, and that perhaps that was how it controlled spindle pole body insertion," says Jaspersen.
To test their idea, the researchers expressed the MPS3-G186K mutant gene in a collection of yeast mutants, looking for ones that would fix the nuclear membrane defect. They found quite a few, and?as expected -- two had mutations in genes that regulate cellular lipid content. When they treated cells with oleic acid (essentially lard), or by slightly increasing the growth temperature -- presumably increasing membrane fluidity, they were able to suppress the defects.
"The nuclear envelope is not just a passive player but presumably is actively remodeled by Mps3 to accommodate the spindle pole body," explains Jaspersen.
Besides their prominent role in mitosis, centrosomes, the higher eukaryotic equivalent of yeast spindle pole bodies, are required for making primary cilia -- a hair-like appendage present in a single copy on every cell. At the base of primary cilia is a centrosome attached to the cell membrane that in cilia has a unique lipid composition. Jaspersen's group is anxious to see what aspects of their Mps3 findings translate to primary cilia. If parallels exist, the rewards could be significant, since ciliary defects can lead to a number of human diseases -- ranging from congenital heart failure to retinal degeneration.
###
Stowers Institute for Medical Research: http://www.stowers-institute.org
Thanks to Stowers Institute for Medical Research for this article.
This press release was posted to serve as a topic for discussion. Please comment below. We try our best to only post press releases that are associated with peer reviewed scientific literature. Critical discussions of the research are appreciated. If you need help finding a link to the original article, please contact us on twitter or via e-mail.
This press release has been viewed 26 time(s).
Source: http://www.labspaces.net/115329/One_for_you__one_for_me
emergency alert system chelsea handler amber rose alexander the great act alabama football 21 jump street
ScienceDaily (Nov. 17, 2011) ? The removal of rare tumor cells circulating in the blood might be possible with the use of biomolecules bound to dendrimers, highly branched synthetic polymers, which could efficiently sift and capture the diseased cells, according to new research at the University of Illinois at Chicago.
Dendrimers have been used to encapsulate drug molecules and serve as a delivery vehicle, but in the new study they were employed to capture circulating tumor cells by biomimicry -- using nanotechnology to create artificial surfaces much like those in real cells.
"We want to take advantage of what nature gives us," says Seungpyo Hong, lead researcher of the study, published in the journal Angewandte Chemie. "We want to create new biomimetic surfaces that will allow us to remove damaged cells from the blood."
Hong, assistant professor of biopharmaceutical sciences at UIC, and his coworkers created a highly sensitive surface that enables multivalent binding -- the simultaneous binding of many molecules to multiple receptors in a biological system. The biomimetic surface was created using dendrimers of seventh-generation polyamidoamine, or PAMAM, and the anti-epithelial cell adhesion molecule, or aEpCAM.
In the body, cancer cells can detach from a primary tumor and flow throughout the bloodstream, enabling them to seed distant new tumors. Rare and difficult to capture, only a few circulating tumor cells can be found in a milliliter of blood in a cancer patient. By comparison, the same volume of blood contains several million white blood cells and a billion red blood cells, Hong said.
Three breast cancer cell lines were used as circulating tumor cell models, with each used to compare the cell adhesion of the dendrimer surfaces to a linear polymer of polyethylene glycol. PEG is commonly used to bind molecules to improve the safety and efficiency of therapeutics.
The nano-scale PAMAM dendrimers were chosen because their size and surface dimension could accommodate multiple anti-epithelial cell adhesion molecules, Hong said. This enabled the multivalent binding, along with the physiological process of "cell rolling" induced by E-selectin, which mimics the process by which circulating tumor cells are recruited to the endothelia and enhances the surface sensitivity toward tumor cells.
The surface developed by the UIC research team demonstrated up to a million-fold increase in binding strength, and up to 7-fold increase in detection efficiency, as compared to the aEpCAM-coated PEG surface that is the current gold standard for circulating tumor cell detection.
Hong says this is the first study to capture the tumor cells on the surface exploiting the multivalent effect, which is most likely due to the spherical architecture of dendrimers. The research was selected as a "Hot Paper" by Angewandte Chemie and highlighted in Faculty of 1000 by Donald Tomalia, the inventor of PAMAM dendrimers.
The results demonstrate that the combination of nanotechnology and biomimicry has a "great potential to be applied for highly sensitive detection of rare tumor cells from blood," Hong said.
Co-authors are David Eddington, associate professor of bioengineering at UIC; and research assistants Ja Hye Myung, Khyati Gajjar and Jelena Saric. The research was funded through a grant from the National Science Foundation.
Recommend this story on Facebook, Twitter,
and Google +1:
Other bookmarking and sharing tools:
Story Source:
The above story is reprinted from materials provided by University of Illinois at Chicago.
Note: Materials may be edited for content and length. For further information, please contact the source cited above.
Journal Reference:
Note: If no author is given, the source is cited instead.
Disclaimer: This article is not intended to provide medical advice, diagnosis or treatment. Views expressed here do not necessarily reflect those of ScienceDaily or its staff.
Source: http://feeds.sciencedaily.com/~r/sciencedaily/~3/jR1UNx1gB9M/111117144049.htm
tesla model s tesla model s prohibition alex honnold how to make it in america how to make it in america nbc news
Source: http://feeds.gawker.com/~r/gizmodo/full/~3/n4qe2vPDetI/why-your-grandchildren-will-never-respect-you
seal beach seal beach bhutan zip code finder zip code finder blackhawks tigers
Deprecated: Assigning the return value of new by reference is deprecated in /home/lern2pla/public_html/external.php on line 526
Deprecated: Assigning the return value of new by reference is deprecated in /home/lern2pla/public_html/external.php on line 619
Deprecated: Assigning the return value of new by reference is deprecated in /home/lern2pla/public_html/external.php on line 621
Deprecated: Assigning the return value of new by reference is deprecated in /home/lern2pla/public_html/external.php on line 652
Deprecated: Assigning the return value of new by reference is deprecated in /home/lern2pla/public_html/external.php on line 697
http://www.lern2play.com Lern2Play.com features a large MMORPG Forum with over 265,000 posts. Active MMORPG and MMO community and MMO Games Forums. Join now to talk about Lord of the Rings Online, World of Warcraft, Age of Conan, Star Wars Galaxies and more.en
Deprecated: Assigning the return value of new by reference is deprecated in /home/lern2pla/public_html/external.php on line 526
Deprecated: Assigning the return value of new by reference is deprecated in /home/lern2pla/public_html/external.php on line 619
Deprecated: Assigning the return value of new by reference is deprecated in /home/lern2pla/public_html/external.php on line 621
Deprecated: Assigning the return value of new by reference is deprecated in /home/lern2pla/public_html/external.php on line 652
Deprecated: Assigning the return value of new by reference is deprecated in /home/lern2pla/public_html/external.php on line 697
http://www.lern2play.com Lern2Play.com features a large MMORPG Forum with over 265,000 posts. Active MMORPG and MMO community and MMO Games Forums. Join now to talk about Lord of the Rings Online, World of Warcraft, Age of Conan, Star Wars Galaxies and more.en
Source: http://feeds.feedburner.com/lern2play
bérénice marlohe bérénice marlohe google offers tim gunn tim gunn death clock death clock
Researchers the world over are seeking reliable ways to predict earthquakes, focusing on identifying seismic precursors that, if detected early enough, could serve as early warnings.
New research, published this week in the journal Applied Physics Letters, suggests that ozone gas emitted from fracturing rocks could serve as an indicator of impending earthquakes. Ozone is a natural gas, a byproduct of electrical discharges into the air from several sources, such as from lightning, or, according to the new research, from rocks breaking under pressure.
Scientists in the lab of Ra?l A. Baragiola, a professor of engineering physics in the University of Virginia School of Engineering and Applied Science set up experiments to measure ozone produced by crushing or drilling into different igneous and metamorphic rocks, including granite, basalt, gneiss, rhyolite and quartz. Different rocks produced different amounts of ozone, with rhyolite producing the strongest ozone emission.
Some time prior to an earthquake, pressures begin to build in underground faults. These pressures fracture rocks, and presumably, would produce detectable ozone.
To distinguish whether the ozone was coming from the rocks or from reactions in the atmosphere, the researchers conducted experiments in pure oxygen, nitrogen, helium and carbon dioxide. They found that ozone was produced by fracturing rocks only in conditions containing oxygen atoms, such as air, carbon dioxide and pure oxygen molecules, indicating that it came from reactions in the gas. This suggests that rock fractures may be detectable by measuring ozone.
Baragiola began the study by wondering if animals, which seem ? at least anecdotally ? to be capable of anticipating earthquakes, may be sensitive to changing levels of ozone, and therefore able to react in advance to an earthquake. It occurred to him that if fracturing rocks create ozone, then ozone detectors might be used as warning devices in the same way that animal behavioral changes might be indicators of seismic activity.
He said the research has several implications.
"If future research shows a positive correlation between ground-level ozone near geological faults and earthquakes, an array of interconnected ozone detectors could monitor anomalous patterns when rock fracture induces the release of ozone from underground and surface cracks," he said.
"Such an array, located away from areas with high levels of ground ozone, could be useful for giving early warning to earthquakes."
He added that detection of an increase of ground ozone might also be useful in anticipating disasters in tunnel excavation, landslides and underground mines.
###
University of Virginia: http://www.virginia.edu
Thanks to University of Virginia for this article.
This press release was posted to serve as a topic for discussion. Please comment below. We try our best to only post press releases that are associated with peer reviewed scientific literature. Critical discussions of the research are appreciated. If you need help finding a link to the original article, please contact us on twitter or via e-mail.
This press release has been viewed 32 time(s).
Source: http://www.labspaces.net/115335/Ozone_from_rock_fracture_could_serve_as_earthquake_early_warning
cadillac xts rambus rambus pabst blue ribbon pabst blue ribbon black friday 2011 mac miller
The Arizona Supreme Court dealt Republican Gov. Jan Brewer a blow Thursday, overturning her decision to oust the chairwoman of the state's independent redistricting commission.
The Arizona Republic reported that the court found that Brewer's removal of Colleen Coyle Mathis did not meet state constitution guidelines.
Brewer removed Mathis on Nov. 2, with the consent of the Republican-controlled Senate, for holding too many secret meetings and for drafting a map that Brewer said did not meet guidelines for compact districts and keeping together "communities of interest."
In court papers, Brewer had argued that Mathis did not use a grid format, as specified in the state constitution, for creating the districts. In addition, Brewer argued that Mathis, a registered independent from Tucson, did not disclose her husband's involvement in the campaign of a Democratic state legislator in her application for the commission.
Brewer's spokesman said it is possible that the governor could seek Mathis' removal at a future date.
"The governor strongly disagrees with the decision," spokesman Matthew Benson said.He said the governor, who was in Washington, D.C., on Thursday, will meet with legislative leaders to consider options. He said the court's order, which he described as "sparse," potentially leaves the door open for the governor to return with a more-specific letter spelling out reasons for removal.
Brewer made the move, which many Democrats had said was a "naked power play," after the state's Republican congressional delegation had lobbied against the draft map released by the redistricting commission. The draft map contained four Republican leaning seats, two Democratic leaning seats and three toss-up seats.
Arizona Democrats had said that Brewer made the decision following a call from Marilyn Quayle, the wife of former Vice President Dan Quayle and mother of Rep. Ben Quayle (R-Ariz.), a charge that Mrs. Quayle has denied. Ben Quayle and Rep. David Schweikert (R-Ariz.) were both placed in the same district in the draft map.
The court's decision was rumored in the days preceding the decision, which came after oral arguments Thursday afternoon in Phoenix. Several sources told The Huffington Post that they expected the court to reinstate Mathis, but also suggested that it would be possible for Brewer to remove her in the future with more specific charges.
Arizona Democrats are cheering the court's decision.
"I was pleased with the order of the court," said State Rep. Matt Heinz (D-Tucson), who was in the courtroom for Thursday's hearing. "I believe it continues the independence of this constitutional branch of government, the independent commission. The attempt of the Republican caucus to hijack the independence of this commission was ghastly."
'; var coords = [-5, -72]; // display fb-bubble FloatingPrompt.embed(this, html, undefined, 'top', {fp_intersects:1, timeout_remove:2000,ignore_arrow: true, width:236, add_xy:coords, class_name: 'clear-overlay'}); });Source: http://www.huffingtonpost.com/2011/11/17/jan-brewer-redistricting_n_1100595.html
joe paterno scandal joe paterno scandal election day 2011 mississippi personhood herman cain press conference joe frazier dead